THE EFFECT OF SALICYLIC ACID ON IN-VITRO GROWTH OF DENDROBIUM MACROPHYLLUM

Abstrak

Dendrobium macrophyllum, has a slow vegetative phase, posing challenges for orchid farmer and enthusiasts. In vitro tissue culture methods can overcome propagation and cultivation issues. Salicylic acid, being a phenolic compound plays a role in regulating plant growth and development in general. This research aimed to evaluate the effect of adding plant growth regulator (salicylic acid), to Vacin-Went (VW) medium on the growth of D. macrophyllum. The method used was Completely Randomized Design consisting of six salicylic acid concentration (0, 1, 2, 3, 5, 7 ppm) with 5 replications, each replication consisting of 7 plantlets. The observed parameters were plant height, number of leaves, plantlets weight, number of roots, roots length, the greenness of the leaves, the roots anatomy, and the leaves anatomy. The data analyzed with analysis of variance (Anova) at significance rate 5% level followed by Duncan’s Multiple Range Test (DMRT) and regression test. The result showed that the addition of 5 ppm salicylic acid to VW medium, significantly enhancesgrowth of plant height, plantlet weight, number of roots, root length, the greenness of the leaves. Salicylic acid also effected the thickness of the anatomical structures of D. macrophyllum roots and leaves.