UJI AKTIVITAS ANTIBAKTERI EKSTRA N-HEKSANA KULIT BATANG BAKAU LINDUR (BRUGUIERA GYMNORRHIZA) TERHADAP STAPHYLOCOCCUS AUREUS

Abstrak

Pendahuluan: Staphylococcus aureus merupakan salah satu penyebab tersering infeksi. Kasus resistansi antibiotik terus mengalami peningkatan, sehingga pengobatan alternatif infeksi bakteri dari bahan dasar tanaman seperti kulit batang Bruguiera gymnorrhiza sangat diperlukan. Tujuan: Mengetahui efek antibakteri ekstrak n-heksana kulit batang Bruguiera gymnorrhiza terhadap Staphylococcus aureus. Metode Penelitian: Desain penelitian eksperimental laboratorik dengan metode sumuran pada media Mueller Hinton Agar. Terdapat 7 kelompok yaitu kontrol positif berupa klindamisin, kontrol negatif berupa akuades, ekstrak n-heksana dengan konsentrasi 25%, 50%, 70%, 90% dan 100%. Tiap kelompok dilakukan pengulangan sebanyak 4 kali. Data diperoleh dari pengukuran diameter zona hambat yang terbentuk di sekitar sumuran. Data diuji dengan Kruskal-Wallis. Hasil: Dari hasil penelitian ini, diperoleh rata-rata diameter zona hambat yaitu 25% dan 50% (0 mm), 70% (4,58 mm), 90% (9,35 mm). 100% (9,91 mm). Kesimpulan: Terdapat efek antibakteri ekstrak n-heksana kulit batang Bruguiera gymnorrhiza terhadap bakteri Staphylococcus aureus.

Introduction: Staphylococcus aureus is one of the most common cause of infections. The treatment for Staphylococcus aureus infections typically involves the use of antibiotics. The incidence of antibiotic resistance continues to rise, therefore the need for alternative treatments using plant-based materials such as the bark of Bruguiera gymnorrhiza is necessary. Purpose: To determine the antibacterial effect of n-hexane of Bruguiera gymnorrhiza bark against Staphylococcus aureus. Method: The research design is a laboratory experimental study using the wall diffusion method on Mueller Hinton Agar. There are 7 groups, including a positive control with clindamycin, a negative control with distilled water, n-hexane extracts at concentrations of 25%, 50%, 70%, 90%, and 100%. Each group was tested in 4 repetitions. The data were obtained by measuring the diameter of the inhibition zone formed around the wells. The data were analyzed using the Kruskal-Wallis test. Result: The results of this study showed that the average diameter of the inhibition zones were 25% and 50% (0 mm), 70% (4,58 mm), 90% (9,35 mm), and 100% (9,91 mm). Conclusion: There was an antibacterial effect n-hexane extracts from the bark of Bruguiera gymnorrhiza against Staphylococcus aureus.