PEMERIKSAAN JUMLAH DAN IDENTIFIKASI MIKROBA UDARA DI LABORATORIUM FAKULTAS SAINS UNIVERSITAS ISLAM NEGERI SULTAN MAULANA HASANUDIN BANTEN

Abstrak

Pemenuhan kualitas udara laboratoium yang baik perlu dilakukan untuk mencegah kontaminasi mikroba, sehingga tidak menyebabkan masalah kesehatan. Penelitian ini bertujuan untuk menentukan jumlah dan mengidentifikasi mikroba udara di Laboratorium Fakultas Sains Universitas Islam Negeri Sultan Maulana Hasanuddin Banten. Pengambilan sampel mikroba dilakukan di 7 ruang laboratorium dan 2 alat kerja Laminar Air Flow (LAF). Jumlah mikroba udara dihitung dalam satuan Coloni Forming Unit Per Meter Kubik (CFU/m3). Identifkasi bakteri dilakukan dengan perwarnaan Gram serta uji biokimia dan identifikasi fungi dilakukan dengan perwarnaan Lactophenol Cotton Blue. Hasil pemeriksaan mikroba udara bakteri menunjukan jumlah mikroba udara pada seluruh laboratorium dan alat 2-54 CFU/ m3, sedangkan fungi sejumlah 1-26 CFU/ m3. Jumlah ini memenuhi persyaratan sesuai Permenaker No.5 Tahun 2018 (Hlm 149 : Standar Faktor Biologis). Pada Permenaker disebutkan batas maksimum keberadaan mikroba bakteri <700 CFU/m3 dan untuk mikroba fungi <1000 CFU/m3. Hasil identifikasi bakteri ditemukan 4 isolat teridentifikasi Bacillus, 2 isolat teridentifikasi Shigella, 1 isolat teridentifikasi Escherichia coli, dan 1 isolat teridentifikasi Staphylococcus. Hasil identifikasi fungi ditemukan 2 isolat teridentifikasi Aspergillus, 1 isolat teridentifikasi Candida, dan 1 isolat tidak dapat diidentifikasi.

Fulfillment of good laboratory air quality is necessary to prevent microbial contamination, so it does not cause health problems. This study aims to determine the number and identify air microbes in the Laboratory of the Faculty of Science, Sultan Maulana Hasanuddin State Islamic University, Banten. Microbial sampling was carried out in 7 laboratory rooms and 2 Laminar Air Flow (LAF) work tools. The number of air microbes was calculated in Coloni Forming Units Per Cubic Meter (CFU/m3). Bacterial identification was carried out by Gram staining and biochemical tests and fungal identification was carried out by Lactophenol Cotton Blue staining. The results of the bacterial air microbial examination showed the number of air microbes in all laboratories and tools was 2-54 CFU/m3, while fungi were 1-26 CFU/m3. This number meets the requirements according to Minister of Manpower Regulation No. 5 of 2018 (Page 149: Biological Factor Standards). The Minister of Manpower Regulation states that the maximum limit for the presence of bacterial microbes is <700 CFU/m3 and for fungal microbes <1000 CFU/m3. The bacterial identification results found 4 isolates identified as Bacillus, 2 isolates identified as Shigella, 1 isolate identified as Escherichia coli, and 1 isolate identified as Staphylococcus. The fungal identification results found 2 isolates identified as Aspergillus, 1 isolate identified as Candida, and 1 isolate could not be identified.